5X9Q

Crystal structure of HldC from Burkholderia pseudomallei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-06
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97927
Spacegroup name	I 1 2 1
Unit cell lengths	74.890, 119.370, 76.703
Unit cell angles	90.00, 110.81, 90.00

Refinement procedure

Resolution	34.592 - 2.400
R-factor	0.2172
Rwork	0.216
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.419
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.5.28)
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.900	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.064	0.160
Number of reflections	46606	1938
<I/σ(I)>	12.5
Completeness [%]	94.7	76.2
Redundancy	3.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.1M MES pH 6.5, 15% (w/w) PEG 3350, 0.56mM CYMAL-6