5X8Y
A Mutation identified in Neonatal Microcephaly Destabilizes Zika Virus NS1 Assembly in vitro
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.934, 100.808, 110.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.652 - 2.817 |
| R-factor | 0.2148 |
| Rwork | 0.210 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o6c |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.691 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.134 | 0.653 |
| Rpim | 0.043 | |
| Number of reflections | 8937 | |
| <I/σ(I)> | 15.9 | 2.8 |
| Completeness [%] | 96.4 | 96.4 |
| Redundancy | 9.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES monohydrate pH 6.0, 14% (w/v) Polyethylene glycol monomethyl ether 2,000, 18% (v/v) 2-Propanol |
