5X8N

Crystal structure of mouse importin-alpha1 bound to the nuclear localization signal of Epstein-Barr virus EBNA-LP protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-02-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	78.270, 90.290, 98.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.765 - 2.150
R-factor	0.1865
Rwork	0.185
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wum
RMSD bond length	0.003
RMSD bond angle	0.656
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.31)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.770	30.770	2.220
High resolution limit [Å]	2.150	8.860	2.150
Rmerge	0.086	0.034	0.964
Rmeas	0.095	0.038	1.060
Rpim	0.039	0.017	0.436
Total number of observations	227759
Number of reflections	38548
<I/σ(I)>	13.1
Completeness [%]	100.0	98.1	100
Redundancy	5.9	5	5.8
CC(1/2)	0.998	0.999	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	MES, sodium citrate, DTT