5X8L

PD-L1 in complex with atezolizumab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	92.197, 169.793, 206.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.921 - 3.100
R-factor	0.2101
Rwork	0.208
R-free	0.25580
RMSD bond length	0.008
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.100
Number of reflections	57608
<I/σ(I)>	6.9
Completeness [%]	97.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Sodium HEPES pH 7.5, 20% w/v PEG8000