5X7U

Trehalose synthase from Thermobaculum terrenum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	I 41 2 2
Unit cell lengths	159.176, 159.176, 152.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.794 - 2.501
R-factor	0.1704
Rwork	0.168
R-free	0.20880
RMSD bond length	0.008
RMSD bond angle	0.966
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	34034
<I/σ(I)>	63.6
Completeness [%]	100.0
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	293	lithium sulfate monohydrate, 0.1 M Tris pH 8.5