5X7K
Crystal structure of the nucleotide-binding domain (NBD) of LipB, a ABC transporter subunit of a type I secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2010-01-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.302, 61.298, 88.313 |
| Unit cell angles | 90.00, 104.03, 90.00 |
Refinement procedure
| Resolution | 35.630 - 2.650 |
| R-factor | 0.20752 |
| Rwork | 0.204 |
| R-free | 0.26133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.081 | 0.497 |
| Number of reflections | 21754 | 996 |
| <I/σ(I)> | 18.05 | 1.53 |
| Completeness [%] | 98.7 | 90.5 |
| Redundancy | 4.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 1.4M K2HPO4, 0.6M NaH2PO4, 0.1M imidazole pH 8.0, 0.2M NaCl |
