5X71

Crystal structure of Rice Dwarf Virus P5 in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-18
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	76.814, 122.631, 192.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.304 - 2.568
R-factor	0.193
Rwork	0.191
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x6x
RMSD bond length	0.004
RMSD bond angle	0.650
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.101	0.507
Number of reflections	57936	2839
<I/σ(I)>	18.9	3.8
Completeness [%]	100.0	99.9
Redundancy	7.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1mM GpppG, 5mM Magnesium chloride, 16% PEG 3350, 0.1M MES, pH 6.0