5X70
Crystal structure of Rice Dwarf Virus P5 in complex with a ssRNA oligomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.754, 80.657, 145.734 |
| Unit cell angles | 104.74, 104.00, 95.29 |
Refinement procedure
| Resolution | 38.527 - 3.300 |
| R-factor | 0.2228 |
| Rwork | 0.220 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x6x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.084 | 0.435 |
| Number of reflections | 50777 | 2528 |
| <I/σ(I)> | 13.3 | 2.3 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350, 0.1M MES, pH 6.0 |
