5X6L
Crystal structure of Notothenia coriiceps adenylate kinase variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 105.444, 105.444, 83.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.156 - 1.862 |
R-factor | 0.1802 |
Rwork | 0.179 |
R-free | 0.20630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5x6k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.180 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.621 | |
Number of reflections | 40301 | |
<I/σ(I)> | 21.34 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2M lithium sulfate, 0.1M acetate pH4.0, 45% PEG 400 |