5X6B

Crystal structure of SepCysE-SepCysS in complex with tRNACys from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	107.252, 107.252, 551.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.221 - 2.596
R-factor	0.2263
Rwork	0.225
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wkr 1b23
RMSD bond length	0.005
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.221
High resolution limit [Å]	2.596
Rmerge	0.097
Number of reflections	59370
<I/σ(I)>	18.4
Completeness [%]	99.7
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5	293	0.1M MES pH 5.0, 2.4M Ammonium Sulfate