5X6A
Crystal structure of an endoglucanase PMO-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-01-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.054, 48.926, 54.192 |
| Unit cell angles | 75.55, 88.29, 76.51 |
Refinement procedure
| Resolution | 27.500 - 1.700 |
| R-factor | 0.13607 |
| Rwork | 0.135 |
| R-free | 0.16585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zud |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.576 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.117 | 0.476 |
| Rpim | 0.062 | 0.242 |
| Number of reflections | 46666 | 2194 |
| <I/σ(I)> | 37.3 | 16.5 |
| Completeness [%] | 95.9 | 95.5 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Magnesium chloride hexahydrate, 25% w/v Polyethylene glycol 3350, 0.1M BIS-TRIS pH 6.0 |
