5X5M

Crystal structure of a hydrolase encoded by lin2189 from Listeria innocua

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97791
Spacegroup name	C 1 2 1
Unit cell lengths	119.630, 49.295, 80.361
Unit cell angles	90.00, 124.98, 90.00

Refinement procedure

Resolution	44.039 - 1.211
R-factor	0.1807
Rwork	0.180
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b49
RMSD bond length	0.006
RMSD bond angle	1.146
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.210
Rmerge	0.061
Rpim	0.025
Number of reflections	112031
<I/σ(I)>	30.5
Completeness [%]	96.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	PEG 4000, Glycerol, MES/imidazole