5X5L
Crystal structure of response regulator AdeR DNA binding domain in complex with an intercistronic region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-11-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.510, 71.380, 78.360 |
| Unit cell angles | 101.96, 104.53, 101.86 |
Refinement procedure
| Resolution | 43.821 - 2.750 |
| R-factor | 0.27933 |
| Rwork | 0.278 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x5j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.821 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 27914 |
| <I/σ(I)> | 9.97 |
| Completeness [%] | 98.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium citrate dibasic, 20% PEG 3350 |
