5X5I
The X-ray crystal structure of a TetR family transcription regulator RcdA involved in the regulation of biofilm formation in Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.477, 75.967, 110.051 |
| Unit cell angles | 90.00, 90.01, 89.95 |
Refinement procedure
| Resolution | 37.983 - 2.554 |
| R-factor | 0.2013 |
| Rwork | 0.198 |
| R-free | 0.26650 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.393 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.084 | 0.292 |
| Number of reflections | 79510 | |
| <I/σ(I)> | 47 | |
| Completeness [%] | 98.8 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4% PEG 400, 0.1M HEPES (pH 7.5), 0.2M L-proline |
