5X57
Structure of GAR domain of ACF7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 263 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.040, 45.990, 25.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.838 - 1.450 |
| R-factor | 0.1301 |
| Rwork | 0.126 |
| R-free | 0.16520 |
| Structure solution method | MIR |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.780 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.840 | |
| High resolution limit [Å] | 1.450 | |
| Rmerge | 0.114 | |
| Number of reflections | 16069 | |
| <I/σ(I)> | 30 | 3.5 |
| Completeness [%] | 98.0 | 97.9 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | Sodium cacodylate trihydrate, (NH4)2SO4, PEG 4000 |
