5X55

Crystal structure of mimivirus uracil-DNA glycosylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	96.267, 95.618, 132.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.744 - 2.302
R-factor	0.1753
Rwork	0.173
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cxm
RMSD bond length	0.008
RMSD bond angle	0.987
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2313: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.119	0.573
Rpim	0.047
Number of reflections	27418
<I/σ(I)>	26.4	5.3
Completeness [%]	99.9	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	HEPES, PEG3350, Isopropanol, CaCl2