5X4G
Crystal structure of Fab fragment of anti-CD147 monoclonal antibody 6H8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 40.104, 100.448, 108.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.339 - 1.450 |
| R-factor | 0.2074 |
| Rwork | 0.206 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.340 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.652 | |
| Number of reflections | 77443 | |
| <I/σ(I)> | 10.9 | 3 |
| Completeness [%] | 98.5 | 95.9 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.16M NaSCN, 26% PEG3350 |
