5X49

Crystal Structure of Human mitochondrial X-prolyl Aminopeptidase (XPNPEP3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	P 1 21 1
Unit cell lengths	62.897, 135.103, 67.205
Unit cell angles	90.00, 99.87, 90.00

Refinement procedure

Resolution	30.983 - 1.650
R-factor	0.1552
Rwork	0.154
R-free	0.17750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wl9
RMSD bond length	0.010
RMSD bond angle	1.069
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.062	0.636
Number of reflections	130822
<I/σ(I)>	12.9	2.2
Completeness [%]	98.8	99.8
Redundancy	3.7	3.7
CC(1/2)	0.998	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		294	10 mM TrisCl, 100 mM NaCl, 50 mM HEPES, 10% PEG 3350, 2.5 mM Apstatin