5X3X
2.8A resolution structure of a cobalt energy-coupling factor transporter-CbiMQO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-10 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.676, 220.564, 301.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.334 - 2.788 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.24860 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.771 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.780 |
| Rmerge | 0.113 |
| Number of reflections | 51793 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 96.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1M ammonium sulfate, 0.1M ADA, pH6.5 |
