5X3P

Crystal structure of the UBX domain of human UBXD7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	32.067, 78.038, 44.963
Unit cell angles	90.00, 102.67, 90.00

Refinement procedure

Resolution	38.240 - 1.999
R-factor	0.1769
Rwork	0.174
R-free	0.22620
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.175
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.240
High resolution limit [Å]	1.990
Rmerge	0.108
Rpim	0.048
Number of reflections	14643
<I/σ(I)>	16.2
Completeness [%]	99.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.1M Tris pH 8.8, 35% v/v Polyethylene glycol 400, 0.1M LiCl