5X2V

Crystal structure of Pseudomonas putida methionine gamma-lyase wild type without sulfate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-01-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	154.219, 153.535, 80.579
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.21907
Rwork	0.216
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o7c
RMSD bond length	0.013
RMSD bond angle	1.589
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.750	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.157	0.388
Number of reflections	75515
<I/σ(I)>	7	3.4
Completeness [%]	97.9	91.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	0.2 M Na-K phosphate buffer, 6-10 % PEG 6000, 0.25 M ammonium sulfate. 0.5 mM PLP