5X2G

Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACC PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	104.345, 105.094, 136.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.547 - 2.400
R-factor	0.191
Rwork	0.190
R-free	0.22130
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	136.520
High resolution limit [Å]	2.400
Rpim	0.043	0.529
Number of reflections	59407
<I/σ(I)>	13.8
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.998	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12.0-14.5% PEG 2000, 0.4M ammonium acetate