5X1U

Structure of the cytosolic domain of DotM derived from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	50.529, 72.021, 65.691
Unit cell angles	90.00, 102.15, 90.00

Refinement procedure

Resolution	40.736 - 1.800
R-factor	0.1764
Rwork	0.176
R-free	0.19830
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.829
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.079	0.077	0.179
Rmeas	0.088	0.084	0.213
Rpim	0.036	0.032	0.111
Total number of observations	169586
Number of reflections	70306
<I/σ(I)>	9.1
Completeness [%]	91.0	99.4	72
Redundancy	4.3	6.2	2.6
CC(1/2)		0.996	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100mM Tris-HCl (pH8.5), 17%(v/v) PEG3350, 200mM MgCl2