5X1E
Structure of DotL(656-783)-IcmS-IcmW derived from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.597, 75.803, 150.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.756 - 1.999 |
R-factor | 0.2231 |
Rwork | 0.221 |
R-free | 0.26900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.972 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.100 | 0.074 | 0.289 |
Total number of observations | 262793 | ||
Number of reflections | 97546 | ||
<I/σ(I)> | 16.7 | ||
Completeness [%] | 92.0 | 98.9 | 85.5 |
Redundancy | 5.4 | 7.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25%(v/v) PEG550 monoethylether, 100mM MES (pH 6.5), 10mM zinc sulfate and 5mM spermine tetrahydrochloride |