5X1D

Crystal Structure of T246A-N247A Human CRMP-2 Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	114.231, 114.231, 193.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.512 - 2.200
R-factor	0.1604
Rwork	0.159
R-free	0.18960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.777
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	32871
<I/σ(I)>	4.9
Completeness [%]	100.0
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 6000, tri-sodium citrate pH 5.6, Lithium sulfate