5X1A

Crystal Structure of Human CRMP-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-25
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	114.121, 114.121, 196.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.344 - 1.821
R-factor	0.1882
Rwork	0.187
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.820
Number of reflections	57910
<I/σ(I)>	9.5
Completeness [%]	99.9
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 8000, Tris pH 8.5, DTT