5X17
Crystal structure of murine CK1d in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.135, 130.349, 53.177 |
| Unit cell angles | 90.00, 115.45, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18996 |
| Rwork | 0.187 |
| R-free | 0.24155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uys |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.667 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.596 | |
| Number of reflections | 41687 | 2087 |
| <I/σ(I)> | 15.3 | 2.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 100 mM Na-citrate, 25 mM (NH4)2SO4 and 7 % PEG 8000, pH 5.0 |
