5X15

Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase ES inhibitor RraA protein family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2823
Spacegroup name	P 32 2 1
Unit cell lengths	96.914, 96.914, 166.556
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.457 - 3.094
R-factor	0.2473
Rwork	0.244
R-free	0.30080
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.620
Data reduction software	DENZO
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.090	3.100
Rmerge	0.084	0.351
Number of reflections	13795	826
<I/σ(I)>	36.4	2.1
Completeness [%]	98.3	98.6
Redundancy	8.6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	287	0.1M NaCl, 0.1M bis-Tris propane, PEG 1500