5X11

Crystal structure of Bacillus subtilis PadR in complex with operator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	341.807, 61.567, 120.553
Unit cell angles	90.00, 107.94, 90.00

Refinement procedure

Resolution	30.000 - 2.650
R-factor	0.23012
Rwork	0.228
R-free	0.26819
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x12
RMSD bond length	0.008
RMSD bond angle	1.274
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.064	0.521
Number of reflections	69940
<I/σ(I)>	31.1
Completeness [%]	99.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	1.4 M ammonium sulfate, 0.1 M MES, 0.2 M lithium sulfate