5X0Z

Crystal structure of FliM-SpeE complex from H. pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	184.314, 184.314, 157.083
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.050 - 2.700
R-factor	0.179
Rwork	0.177
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cmg 4gc8
RMSD bond length	0.009
RMSD bond angle	1.417
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((DEV_2621: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	83101
<I/σ(I)>	23.5
Completeness [%]	99.0
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	imidazole/hydrochloric acid, sodium citrate tribasic, ammonium sulfate