5X0V
Reduced form of regulatory domain of OxyR2 from Vibrio vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.204, 138.348, 97.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.736 - 1.600 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19160 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.088 | 0.343 |
| Rpim | 0.027 | 0.164 |
| Number of reflections | 62093 | |
| <I/σ(I)> | 18.6 | 2.45 |
| Completeness [%] | 98.7 | 95.5 |
| Redundancy | 8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 0.2M ammonium citrate (pH 6.5), 14% (w/v) PEG 3350, 2mM TCEP (Tris(2-carboxyethyl)phosphine), 5mM DTT |
