5X0F
Free serine kinase (E30A mutant) in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-13 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.100 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 39.903, 70.032, 88.381 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.760 |
R-factor | 0.22496 |
Rwork | 0.223 |
R-free | 0.26097 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5x0b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.541 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.760 |
Number of reflections | 24624 |
<I/σ(I)> | 13.4 |
Completeness [%] | 97.7 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | PEG 3350, serine |