5X09
Crystal structure of subunit A mutant P235A/S238C of the A-ATP synthase from pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.467, 127.467, 105.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.23707 |
| Rwork | 0.235 |
| R-free | 0.27239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m4y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.130 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 36816 | |
| <I/σ(I)> | 24.07 | 2.83 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.4 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 50%(v/v) MPD, 0.1M acetate buffer pH 4.5 |
