5WZP
Alpha-N-acetylgalactosaminidase NagBb from Bifidobacterium bifidum - ligand free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.637, 127.764, 176.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.550 - 2.640 |
| R-factor | 0.23453 |
| Rwork | 0.231 |
| R-free | 0.31098 |
| Structure solution method | SAD |
| RMSD bond length | 0.037 |
| RMSD bond angle | 1.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.550 | 2.700 |
| High resolution limit [Å] | 2.640 | 2.650 |
| Rmerge | 0.086 | 0.627 |
| Number of reflections | 42830 | |
| <I/σ(I)> | 18.3 | 1.9 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.02M MgCl2, 0.1M HEPES-NaOH, 7.5% PEG 3350 |
