5WZP

Alpha-N-acetylgalactosaminidase NagBb from Bifidobacterium bifidum - ligand free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	63.637, 127.764, 176.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.550 - 2.640
R-factor	0.23453
Rwork	0.231
R-free	0.31098
Structure solution method	SAD
RMSD bond length	0.037
RMSD bond angle	1.713
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.550	2.700
High resolution limit [Å]	2.640	2.650
Rmerge	0.086	0.627
Number of reflections	42830
<I/σ(I)>	18.3	1.9
Completeness [%]	99.6	99.2
Redundancy	6.9	6.6
CC(1/2)		0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.02M MgCl2, 0.1M HEPES-NaOH, 7.5% PEG 3350