5WZJ

Structure of APUM23-GGAUUUGACGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 65
Unit cell lengths	188.319, 188.319, 45.383
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.115 - 2.101
R-factor	0.1771
Rwork	0.176
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wzg
RMSD bond length	0.007
RMSD bond angle	0.829
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.088	0.047	0.531
Rmeas	0.091	0.049	0.549
Rpim	0.021	0.011	0.135
Number of reflections	54262
<I/σ(I)>	6.8
Completeness [%]	100.0	99.5	100
Redundancy	18.5	18	16.3
CC(1/2)		0.999	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2M Sodium malonate (pH 7.0), 20% w/v Polyethylene glycol 3350