5WZE

The structure of Pseudomonas aeruginosa aminopeptidase PepP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	111.197, 123.432, 149.485
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.008 - 1.783
R-factor	0.2106
Rwork	0.210
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1AZ9
RMSD bond length	0.011
RMSD bond angle	0.997
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	1.827
High resolution limit [Å]	1.783	1.783
Rmerge		0.709
Number of reflections	194517	18600
<I/σ(I)>	19.714
Completeness [%]	99.0
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	30% PEG400, 100 mM sodium cacodylate pH 6.5, 200 mM lithium sulfate