5WZ3
Crystal structure of Zika virus NS5 RNA-dependent RNA polymerase(RdRP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.72929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.528, 84.851, 69.199 |
Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
Resolution | 34.159 - 1.804 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.20610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j7u |
RMSD bond length | 0.005 |
RMSD bond angle | 0.958 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.078 |
Number of reflections | 61460 |
<I/σ(I)> | 22.098 |
Completeness [%] | 98.5 |
Redundancy | 6.1 |
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 20 mM D-glucose, 20 mM D-mannose, 20 mM D-galactose, 20 mM L-fucose, 20 mM D-xylose, 20 mM N-acetyl-D-glucosamine, 60.9 mM Tris (base), 39.1 mM Bicine, pH 8.5, 12 % v/v ethylene glycol and 6 % w/v PEG 8000 |