5WYX
Crystal structure of human TLR8 in complex with CU-CPT8m
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2016-02-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.144, 99.149, 140.247 |
Unit cell angles | 90.00, 108.07, 90.00 |
Refinement procedure
Resolution | 40.164 - 2.400 |
R-factor | 0.20343 |
Rwork | 0.201 |
R-free | 0.25628 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.907 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 144258 |
<I/σ(I)> | 30 |
Completeness [%] | 99.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000, calcium chloride, Tris-HCl, ethylene glycol |