5WYQ
Crystal Structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 85.896, 85.896, 147.838 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.120 - 2.160 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p9p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.120 | 2.290 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.057 | 0.800 |
Number of reflections | 34505 | 2943 |
<I/σ(I)> | 15.9 | 1.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Tris-Cl pH 8.4, 12% MPD, 12% PEG 1000, 12% PEG 3350, 5% PEG 300 |