5WYH

Crystal structure of RidL(1-200) complexed with VPS29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-12
Detector	MAR CCD 130 mm
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	44.312, 82.049, 87.332
Unit cell angles	101.73, 104.26, 104.77

Refinement procedure

Resolution	31.860 - 2.460
R-factor	0.19131
Rwork	0.190
R-free	0.22494
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.392
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.106	0.067	0.891
Rmeas	0.116	0.078
Rpim	0.063	0.040	0.534
Total number of observations	138440
Number of reflections	37098
<I/σ(I)>	10.3
Completeness [%]	96.6	94.8	96.7
Redundancy	3.7	3.8	3.7
CC(1/2)		0.993	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris pH8.0, 20% PEG 8000