5WY2

Human Snx5 PX domain in complex with Chlamydia IncE C terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-12
Detector	Nonius Kappa CCD
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	37.443, 43.711, 60.070
Unit cell angles	94.79, 99.81, 100.97

Refinement procedure

Resolution	50.010 - 1.900
R-factor	0.18912
Rwork	0.187
R-free	0.22274
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hpc
RMSD bond length	0.014
RMSD bond angle	1.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.071	0.049	0.859
Rmeas	0.096	0.069
Rpim	0.069	0.049	0.859
Number of reflections	27655		1378
<I/σ(I)>	8.8
Completeness [%]	96.6	98	95.8
Redundancy	2	2	2
CC(1/2)		0.987	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium acetate, 0.1M Sodium acetate pH 4.6, 30% w/v Polyethylene glycol 4000