5WXY
Crystal structure of Microcystis aeruginosa PCC 7806 aspartate racemase in complex with L-aspartate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97791 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 128.482, 128.482, 45.143 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.831 - 2.630 |
| R-factor | 0.18708 |
| Rwork | 0.186 |
| R-free | 0.20527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wxx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.270 | 2.680 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 12847 | |
| <I/σ(I)> | 21.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% (w/v) polyethylene glycol 3350, 0.2 magnesium chloride |
