5WXV

The crystal structure of VabB-ICL domain from Vibrio anguillarum 775

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-14
Detector	MAR CCD 130 mm
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	49.527, 173.882, 143.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.921 - 2.300
R-factor	0.209
Rwork	0.206
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gle
RMSD bond length	0.008
RMSD bond angle	1.018
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.921
High resolution limit [Å]	2.300
Number of reflections	107112
<I/σ(I)>	2.5
Completeness [%]	99.5
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG8000, 0.1M imidazole, 0.2M calcium acetate