5WXU
11S globulin from Wrightia tinctoria reveals auxin binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.209, 114.242, 202.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.240 - 1.700 |
| R-factor | 0.17677 |
| Rwork | 0.174 |
| R-free | 0.22718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehk |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.175 |
| Data reduction software | MxCuBE |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.200 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 270464 | |
| <I/σ(I)> | 16.77 | 2.34 |
| Completeness [%] | 94.2 | 92.43 |
| Redundancy | 3.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Sodium citrate tribasic dihydrate, 100 mM Tris hydrochloride pH 8.5, 30% v/v PEG 400 |
