5WXK
EarP bound with domain I of EF-P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.204, 57.254, 72.831 |
| Unit cell angles | 90.00, 101.44, 90.00 |
Refinement procedure
| Resolution | 41.965 - 1.801 |
| R-factor | 0.1592 |
| Rwork | 0.157 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apo EarP |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.901 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.660 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 45727 | |
| <I/σ(I)> | 12.1 | 1.9 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 4.1 | 4 |
| CC(1/2) | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Hepes-NaOH buffer (pH 7.0) and 1.8 M ammonium sulfate |
