5WXK

EarP bound with domain I of EF-P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	122.204, 57.254, 72.831
Unit cell angles	90.00, 101.44, 90.00

Refinement procedure

Resolution	41.965 - 1.801
R-factor	0.1592
Rwork	0.157
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	apo EarP
RMSD bond length	0.007
RMSD bond angle	0.901
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.660	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	45727
<I/σ(I)>	12.1	1.9
Completeness [%]	99.8	98.3
Redundancy	4.1	4
CC(1/2)		0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Hepes-NaOH buffer (pH 7.0) and 1.8 M ammonium sulfate