5WXD

Crystal Structure of HLA-A*2402 in complex with avian influenza A(H7N9) virus-derived peptide H7-25 (data set 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-15
Detector	SDMS
Wavelength(s)	1.03871
Spacegroup name	P 21 21 21
Unit cell lengths	67.470, 78.983, 88.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.326 - 3.295
R-factor	0.2052
Rwork	0.203
R-free	0.25400
RMSD bond length	0.003
RMSD bond angle	0.681
Data reduction software	CNS
Data scaling software	CNS
Phasing software	CNS
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.295
Number of reflections	7564
<I/σ(I)>	12.175
Completeness [%]	86.4
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20000