5WXC

Crystal Structure of HLA-A*2402 in complex with avian influenza A(H7N9) virus-derived peptide H7-25 (data set 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-15
Detector	SDMS
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	81.373, 67.292, 88.346
Unit cell angles	90.00, 98.43, 90.00

Refinement procedure

Resolution	30.824 - 2.295
R-factor	0.1791
Rwork	0.177
R-free	0.22400
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	CNS
Data scaling software	CNS
Phasing software	CNS
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.295
Number of reflections	42274
<I/σ(I)>	18.155
Completeness [%]	99.1
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4000