5WX8
Human herpesvirus 6A immediate early protein 2 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.840, 39.710, 103.730 |
| Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
| Resolution | 42.508 - 2.500 |
| R-factor | 0.2233 |
| Rwork | 0.220 |
| R-free | 0.27010 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS (Oct 15, 2015) |
| Data scaling software | XDS (Oct 15, 2015) |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.510 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 27197 |
| <I/σ(I)> | 9.47 |
| Completeness [%] | 97.9 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 277 | 24% polyethylene glycol 3350, 0.2M sodium formate, 20 mM Tris-HCl (pH 8.8), 20 mM CaCl2, 1 mM DTT |
