5WX5
Alkylquinolone synthase Y215V mutant from Evodia rutaecarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 186.160, 186.160, 139.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.540 - 3.058 |
| R-factor | 0.1848 |
| Rwork | 0.182 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wx4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.240 |
| High resolution limit [Å] | 3.058 | 3.058 |
| Rmerge | 0.172 | 0.680 |
| Number of reflections | 52842 | |
| <I/σ(I)> | 14.9 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M Sodium citrate, 1.99 M Ammonium sulfate |
