5WWW

Crystal structure of the KH1 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 62
Unit cell lengths	76.366, 76.366, 36.392
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.183 - 1.798
R-factor	0.1738
Rwork	0.172
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dgr
RMSD bond length	0.006
RMSD bond angle	0.778
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.159	0.121	0.705
Rmeas	0.168	0.128	0.742
Rpim	0.053	0.042	0.229
Total number of observations	115295
Number of reflections	11406
<I/σ(I)>	3.6
Completeness [%]	99.6	99.4	98.9
Redundancy	10.1	9.6	10.3
CC(1/2)		0.989	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5% v/v Tacsimate pH 7.0, 0.1M HEPES pH 7.0, 10% w/v polyethylene glycol monomethyl ether 2000